Purpose : To compare color appearance differences under daylight-simulating D65 and an LED6500K light source with the same color temperature, and to examine differences in color discrimination according to age and gender. Methods : A total of 100 adults participated in the Farnsworth-Munsell 100 Hue Test under both light conditions. Total Error Scores (TES) were calculated and analyzed based on light source, age group (20–30s vs 50–60s), and gender. Results : TES of all participants was higher under the LED6500K light source compared with the D65 light source, with individuals in their 20–30s showing greater sensitivity to changes in light source. Across both light sources, participants in their 50–60s demonstrated higher TES, with a statistically more significant age-related increase under D65 light source. Differences by gender were generally not statistically significant. Conclusion : Although color discrimination was lower under LED6500K than D65 light source, LED6500K performed better than conventional indoor lighting and is suitable for indoor use. Color discrimination declines with age, and younger adults showed greater sensitivity to lighting changes. This study provides foundational data on color appearance across demographic groups and may serve as a useful reference for product selection, customer interaction, and optician training in optical practices.