Purpose : This study aimed to examine the impact of night shifts and long working hours on eye fatigue and work-related stress among workers, and to clarify the relationship between work patterns and health outcomes. Methods : This study utilized raw data from the 7th Working Environment Survey (2023) conducted by the Korea Occupational Safety and Health Agency to analyze the relationships between work patterns, eye fatigue, and work-related stress among workers. A significance level of p<0.050 was applied, and age and gender were adjusted to evaluate the independent effects of work patterns on eye fatigue. Results : The analysis showed that night shift workers had a higher risk of eye fatigue compared to non-night shift workers, whereas long working hours were associated with a lower risk of eye fatigue. In addition, the prevalence of eye fatigue significantly increased with higher levels of work-related stress, and night shift workers exhibited relatively higher levels of stress. Conclusion : The study confirmed that work patterns are closely associated with eye fatigue and work-related stress. In particular, night shifts were identified as factors that simultaneously increase the risk of eye fatigue and job stress, whereas long working hours unexpectedly showed a lower risk of eye fatigue, suggesting the influence of work environment characteristics and individual adaptation. These findings highlight the need for tailored management strategies that consider work patterns to promote ocular health and reduce job-related stress among workers.